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pro-q diamond staining  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pro-q diamond staining
    Pro Q Diamond Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pro-q%C2%AE+diamond/pro+q+diamond+stain/pmc12204518-117-12-15
    Average 90 stars, based on 1 article reviews
    pro-q diamond staining - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.

    Magnetic Beads:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.

    Protease Inhibitor:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.

    SDS Page:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.

    Molecular Weight:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.

    Staining:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.

    Imaging:

    Article Title: Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas
    Article Snippet: Phosphorylation was monitored utilizing Pro-Q® Diamond (ThermoFisher).

    Article Title: Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis
    Article Snippet: We incubated the total cell lysates with anti-FLAG M2 Magnetic Beads (Sigma, Catalog #: SIAL-M8823-1 ML) for 3 h at 4 °C, washed it with Wash Buffer (PBS, 0.0001% NP-40, and protease and phosphatase inhibitors), eluted the proteins in Elution Buffer (0.1 M Glycine-HCl, pH 2.5, protease inhibitor cocktail (Thermo-Fisher, Catalog #: 87786) and the PhosSTOP phosphatase inhibitor (Roche, Catalog #: 04906845001)), added the SDS Loading Buffer, and heated the eluates at 95 °C for 5 min. We performed SDS-PAGE on these samples along with PeppermintStickTM Phosphoprotein Molecular Weight Standards (Thermo-Fisher, Catalog #: P27167), fixed the gel in Fix Solution (50% methanol, 10% acetic acid), stained it with Pro-Q® Diamond (ThermoFisher, Catalog #: P33300) for 70 min to visualize phosphorylated CTNNA3, and captured the image of the stained gel using the ChemiDoc MP Imaging System (Illumination source: Epi-green, 520–545 nm excitation; Filter: 590/110 nm standard filter).

    Article Title: Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis.
    Article Snippet: 2-DE gel staining Following 2-DE, the gels were stained using Pro-Q® Diamond (Molecular Probes, Inc., Eugene, OR, USA) as follows.



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